Search results for " light-sheet microscopy"

showing 3 items of 3 documents

Fast Inertia-Free Volumetric Light-Sheet Microscope

2017

Fast noninvasive three-dimensional (3D) imag-ing is crucial for quantitatively studying highly dynamic events ranging from flow cytometry to developmental biology. Light-sheet microscopy has emerged as the tool-of-choice for 3D characterization of rapidly evolving systems. However, to obtain a 3D image, either the sample or parts of the microscope are moved, limiting the acquisition speed. Here, we propose a novel inertia-free light-sheet-based scheme for volumetric imaging at high temporal resolution. Our approach comprises a novel combination of an acousto-optic scanner to produce tailored illumination and an acoustic-optofluidic lens, placed in the detection path to provide extended dept…

0301 basic medicineScanneracouto-optic devicesMaterials scienceMicroscopethree-dimensional microscopy01 natural sciencesAcouto-optic devices flow cytometry light-sheet microscopy three-dimensional microscopy Electronic Optical and Magnetic Materials Biotechnology Atomic and Molecular Physics and Optics Electrical and Electronic Engineeringlaw.invention010309 optics03 medical and health sciencesOpticslawAtomic and Molecular Physics0103 physical sciencesMicroscopyElectronicOptical and Magnetic MaterialsElectrical and Electronic Engineeringbusiness.industryflow cytometryRangingFrame rateAtomic and Molecular Physics and OpticsElectronic Optical and Magnetic MaterialsCharacterization (materials science)Lens (optics)acouto-optic devices; flow cytometry; light-sheet microscopy; three-dimensional microscopy; Electronic Optical and Magnetic Materials; Biotechnology; Atomic and Molecular Physics and Optics; Electrical and Electronic Engineering030104 developmental biologyLight sheet fluorescence microscopyand Opticsbusinesslight-sheet microscopyBiotechnologyACS Photonics
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Dual-beam confocal light-sheet microscopy via flexible acousto-optic deflector

2019

Confocal detection in digital scanned laser light-sheet fluorescence microscopy (DSLM) has been established as a gold standard method to improve image quality. The selective line detection of a complementary metal-oxide-semiconductor camera (CMOS) working in rolling shutter mode allows the rejection of out-of-focus and scattered light, thus reducing background signal during image formation. Most modern CMOS have two rolling shutters, but usually only a single illuminating beam is used, halving the maximum obtainable frame rate. We report on the capability to recover the full image acquisition rate via dual confocal DSLM by using an acoustooptic deflector. Such a simple solution enables us t…

Image formationPaperMaterials scienceImage qualityConfocalBiomedical Engineeringacousto-optic deflector; confocal detection; digital scanned laser light-sheet fluorescence microscopy; high contrast; high-throughput microscopy; light-sheet microscopy; mouse brain; zebrafish brainconfocal detection01 natural scienceslaw.invention010309 opticsBiomaterialsMiceacousto-optic deflectorOpticslaw0103 physical sciencesMicroscopyImage Processing Computer-AssistedAnimalsZebrafishhigh-throughput microscopyconfocal light-sheet microscopyMicroscopyMicroscopy Confocalbusiness.industryhigh contrastRolling shutterBrainEquipment DesignLaserFrame ratezebrafish brainAtomic and Molecular Physics and OpticsElectronic Optical and Magnetic MaterialsHigh-Throughput Screening AssaysMice Inbred C57BLdigital scanned laser light-sheet fluorescence microscopyMicroscopy FluorescenceLight sheet fluorescence microscopyLarvamouse brainbusinesslight-sheet microscopyJournal of Biomedical Optics
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Removing striping artifacts in light-sheet fluorescence microscopy: a review

2022

Abstract In recent years, light-sheet fluorescence microscopy (LSFM) has found a broad application for imaging of diverse biological samples, ranging from sub-cellular structures to whole animals, both in-vivo and ex-vivo, owing to its many advantages relative to point-scanning methods. By providing the selective illumination of sample single planes, LSFM achieves an intrinsic optical sectioning and direct 2D image acquisition, with low out-of-focus fluorescence background, sample photo-damage and photo-bleaching. On the other hand, such an illumination scheme is prone to light absorption or scattering effects, which lead to uneven illumination and striping artifacts in the images, oriented…

Materials scienceOptical sectioningBiophysicsBrain imaging01 natural sciences010309 optics03 medical and health sciencesOptics0103 physical sciencesFluorescence microscopeAnimalsMolecular Biology030304 developmental biology0303 health sciencesLight-sheet microscopyScatteringbusiness.industryRangingSample (graphics)FluorescenceMicroscopy FluorescenceLight sheet fluorescence microscopy3D microscopyStripingData striping3D microscopy; Brain imaging; Light-sheet microscopy; StripingArtifactsbusinessProgress in Biophysics and Molecular Biology
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